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TPE-Puffer
ββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββ
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TPE-Puffer (Tris-Phosphat-EDTA-Puffer) ist ein Elektrophoresepuffer, der in der Biochemie und Molekularbiologie im Zuge einer Agarose-Gelelektrophorese zur Trennung von NukleinsΓ€uren wie DNA oder RNA verwendet wird.
Contents
β’ Eigenschaften
β’ Einzelnachweise
ββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββββ
Eigenschaften
Der TPE-Puffer ist aus TRIS (89 mM), PhosphorsΓ€ure (1,3 % m/V) und EDTA (2 mM) zusammengesetzt.cite-ref-buckingham-1-0[1] Alternativ verwendete Puffer sind der TAE-Puffer, der TBE-Puffer, der SB-Puffer und der Lithiumborat-Puffer. TPE-Puffer hat eine hΓΆhere PufferkapazitΓ€t als TAE-Puffer,cite-ref-kumar-2-0[2] d. h. die Elektrophorese kann lΓ€nger laufen, ohne dass sich der pH-Wert verΓ€ndert.cite-ref-weising-3-0[3] Bei groΓen DNA-MolekΓΌlen erzeugt es eine bessere Trennung als TAE-Puffer, wΓ€hrend es bei kleinen DNA-MolekΓΌlen zu einer schlechteren Trennung fΓΌhrt.cite-ref-kumar-2-1[2]
Einzelnachweise
cite-note-buckingham-11. β Lela Buckingham: Molecular Diagnostics. F.A. Davis, 2011, ISBN 978-0-8036-2975-2. S. 95.
cite-note-kumar-22. β Anil Kumar: Genetic Engineering. Nova Publishers, 2005, ISBN 978-1-59454-753-9, S. 91.
cite-note-weising-33. β Kurt Weising: DNA Fingerprinting in Plants. CRC Press, 2005, ISBN 978-1-4200-4004-3, S. 116.